Mouse FGF-21 Biotinylated Antibody

Catalog #: BAF3057 Datasheet / COA / SDS

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BAF3057

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All FGF-21 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FGF-21 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF-21 in Western blots. In this format, approximately 5% cross-reactivity with rhFGF-21 is observed and less than 1% cross-reactivity with rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, rmFGF-8b, -15 and -23 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse FGF-21
Tyr30-Ser210
Accession # Q9JJN1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse FGF‑21

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-21

Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.

Long Name

Fibroblast Growth Factor 21

Entrez Gene IDs

26291 (Human); 56636 (Mouse)

Alternate Names

FGF21; FGF-21; fibroblast growth factor 21