Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody

FAB1037V Datasheet / COA / SDS

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FAB1037V-025

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Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody in Data

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Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.

Source

Monoclonal Rat IgG_2B Clone # RB6-8C5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

The immunogen for this antibody is Ly-6G (Gr-1, Gr1).

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of Gr‑1/Ly‑6G in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1037V) or (B) Rat IgG_2BAlexa Fluor 405 Isotype Control (Catalog # IC013V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References