Mouse IL-17F Antibody (1058A)

R&D Systems | Catalog # MAB20571

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1058A
View all IL-17F Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant mouse IL-17F
Ala21-Val153
Accession # Q7TNI7

Specificity

Detects mouse IL-17F in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse IL-17F Antibody (1058A)

Detection of IL‑17F antibody in Mouse Splenocytes Stimulated to Induce Th17 cells antibody by Flow Cytometry.

Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 cells by Flow Cytometry.

Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Applications for Mouse IL-17F Antibody (1058A)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes stimulated to induce Th17 cells

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17F

IL-17F, also called ML-1 or IL-24, is one of six IL-17 family members.  All are homodimeric glycoproteins with conserved cysteines that form a cystine knot structure.  IL-17F is a 44 kDa dimer expressed by activated monocytes that acts in a stimulation loop with activated CD4+T cells to produce IL-2, TGFb and MCP-1.  Like IL-17A, it recruits neutrophils to airways during allergic reactions. IL-17F has a long form and a short form (predominant), which have alternate start sites but similar function. Mouse IL-17F is 58% aa identical to human IL-17F.

Long Name

Interleukin 17F

Alternate Names

IL17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Gene Symbol

IL17F

UniProt

Q7TNI7

Additional IL-17F Products

Product Documents for Mouse IL-17F Antibody (1058A)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-17F Antibody (1058A)

For research use only

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