Mouse IL-2 Antibody (JES6-1A12)

Name: Mouse IL-2 Antibody (JES6-1A12)
Brand: R&D Systems
SKU: MAB702
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # MAB702

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Citations (17)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization, Adoptive Transfer, Bioassay, ELISA Capture, ELISA Development, ELISpot Development, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # JES6-1A12

View all IL-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-2

Specificity

Detects mouse IL-2 in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-2 Antibody (JES6-1A12)

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody.

Recombinant Mouse IL-2 (Catalog # 402-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-2 Monoclonal Antibody (Catalog # MAB702). The ND₅₀ is typically 0.125-0.75 µg/mL.

Mouse IL-2 ELISA Standard Curve

Recombinant Mouse IL‑2 (Catalog # 402-ML) was serially diluted and captured by Rat Anti-Mouse IL‑2 Monoclonal Antibody (Catalog # MAB702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse IL-2 Antibody (JES6-1A12)

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND₅₀) is typically 0.125-0.75 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑2.

Mouse IL-2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse IL‑2 Biotinylated Antibody (Catalog # BAF402)
Standard: Recombinant Mouse IL-2 Protein (Catalog # 402-ML)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-2

IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)