Mouse Integrin  beta 4/CD104 Fluorescein‑conjugated Antibody

R&D Systems | Catalog # FAB4054F

R&D Systems
Discontinued Product
FAB4054F has been discontinued. View all Integrin beta 4/CD104 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

FITC (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 308601
View all Integrin beta 4/CD104 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  beta 4/CD104
Asn29-Ser711
Accession # NP_001005608

Specificity

Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Integrin beta 1, beta 2, beta 6, beta 7, or recombinant human Integrin beta 3.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Integrin  beta 4/CD104 Fluorescein‑conjugated Antibody

Detection of Integrin beta 4/CD104 antibody in CMT-93 Mouse Cell Line antibody by Flow Cytometry.

Detection of Integrin beta 4/CD104 in CMT‑93 Mouse Cell Line by Flow Cytometry.

CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse Integrin beta 4/CD104 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4054F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin  beta 4/CD104 Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CMT‑93 mouse rectal carcinoma cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin beta 4/CD104

Integrin beta 4, also known as CD104, is a 200 kDa type I transmembrane (TM) glycoprotein that associates only with Integrin  alpha 6/CD49f. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22 aa TM segment and an unusually long 1073 aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity.

Alternate Names

CD104, ITGB4

Entrez Gene IDs

3691 (Human); 192897 (Mouse); 25724 (Rat)

Gene Symbol

ITGB4

UniProt

NP_001005608

Additional Integrin beta 4/CD104 Products

Product Documents for Mouse Integrin  beta 4/CD104 Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Integrin  beta 4/CD104 Fluorescein‑conjugated Antibody

For research use only

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