Lefty-2 (Left-right determination factor 2; also Lefty-B [in human]) is a atypical member of the TGF-beta family of proteins. It is expressed during early embryogenesis in both the primitive streak and left-side lateral plate mesoderm. In the adult, Lefty-2 appears in oviduct epithelium. Lefty-2 acts in a manner reminiscent of that for Chordin and Noggin, and it is assumed that Lefty-2 is an antagonist of BMP activity. Notably, Lefty-2 and Nodal are likely under the control of Lefty-1, and thus all three molecules would appear to contribute to the creation of a left side-type body plan. Mouse Lefty-2 is synthesized as a 368 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, plus a 347 aa, 41-42 kDa bioactive proprecursor that may undergo proteolytic processing at one of two downstream cleavage sites. If cleavage occurs after Arg77, the resulting 33-34 kDa mature form (aa 78-368) is biologically inactive; if cleavage occurs after Arg135, the resulting 27-28 kDa mature form (aa 136-368) is biologically active. Lefty-2 is not a covalent homodimer and has been suggested to act as a monomer. Over aa 78-368, mouse Lefty-2 shares 94%, 83% and 95% aa sequence identity with rat Lefty-2, human Lefty-B and mouse Lefty-1, respectively.
Key Product Details
Validated by
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Phe78-Leu368
Accession # P57785
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse Lefty-2 Antibody
Detection of Mouse Lefty-2 by Western Blot.
Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse Lefty-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lefty-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lefty-2 in D3 Mouse Embryonic Stem Cells.
Lefty-2 was detected in immersion fixed D3 mouse embryonic stem cells untreated (lower panel) or stimulated (upper panel) with recombinant human/mouse/rat Activin A (Catalog # 338-AC) using Sheep Anti-Mouse Lefty-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7648) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of Activin A-stimulated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Mouse Lefty-2 Antibody
Immunocytochemistry
Sample: Immersion fixed D3 mouse embryonic stem cells untreated or stimulated with recombinant human/mouse/rat Activin A (Catalog # 338-AC)
Western Blot
Sample: P19 mouse embryonal carcinoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Lefty-2
Alternate Names
Gene Symbol
UniProt
Additional Lefty-2 Products
Product Documents for Mouse Lefty-2 Antibody
Product Specific Notices for Mouse Lefty-2 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars