Mouse LIMPII/SR-B2 Lumenal Loop Antibody

R&D Systems | Catalog # AF1888

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, ELISA Detection

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LIMPII/SR-B2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LIMPII/SR‑B2 Lumenal Loop
Arg27-Thr432
Accession # O35114

Specificity

Detects mouse LIMPII/SR‑B2 Lumenal Loop in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human (rh) LIMPII is observed and less than 1% cross‑reactivity with rhCD36 and rhSR‑B1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Detection of LIMPII/SR-B2 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of LIMPII/SR-B2 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse LIMPII/SR-B2 Affinity-purified Polyclonal Antibody (Catalog # AF1888, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes fixed with paraformaldehyde and permeablized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse LIMPII/SR-B2 Fc Chimera (Catalog # 1888-LM)

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIMPII/SR-B2

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1‑3). SR-B/CD36 family members are type III integral membrane proteins with an N‑ as well as a C-terminal cytoplasmic tail, and a large extracellular (or luminal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is expressed on all tissues and cell types examined so far, including activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII‑thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation and inflammation (1). Overexpression of LIMPII causes an enlargement of early and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

References

  1. Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. 
  2. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
  3. Eskelinen, E-L. et al. (2003) Trends in Cell Biol. 13:137.
  4. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
  5. Gamp, A-C. et al. (2003) Human Mol. Genet. 12:631.

Long Name

Lysosomal Integral Membrane Protein II

Alternate Names

CD36L2, LPG85, SCARB2, SR-B2, SR-BII, SRB2

Entrez Gene IDs

950 (Human); 12492 (Mouse)

Gene Symbol

SCARB2

UniProt

O35114

Additional LIMPII/SR-B2 Products

Product Documents for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Certificate of Analysis

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Product Specific Notices for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

For research use only

Related Research Areas

Monocyte Markers

Citations for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

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