Mouse MDL-1/CLEC5A Antibody (226402)

R&D Systems | Catalog # MAB1639

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 226402
View all MDL-1/CLEC5A Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with mouse MDL-1/CLEC5A

Specificity

Detects mouse MDL-1/CLEC5A. In ELISAs, approximately 50% cross-reactivity with recombinant human MDL‑1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse MDL-1/CLEC5A Antibody (226402)

Detection of MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse MDL-1/CLEC5A Monoclonal Antibody (MAB1639, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins.
Detection of MDL-1/CLEC5A by Western Blot

Detection of MDL-1/CLEC5A by Western Blot

Native gel analysis of type II receptors and ActA, ActAC, and ActC.Native PAGE analysis of ActRIIA (A) and ActRIIB (B) with activin A (ActA) and activin AC (ActAC). Binary complexes were formed by titrating receptor from 1:1 to 3:1 molar ratio against constant ligand. (C) Native PAGE analysis of 1:3 receptor: ligand molar ratio with activin C (ActC). Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/78197), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse MDL-1/CLEC5A Antibody (226402)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.

Long Name

Myeloid DAP12-associating Lectin

Alternate Names

CLEC5A, CLECSF5, MDL1

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Gene Symbol

CLEC5A

Additional MDL-1/CLEC5A Products

Product Documents for Mouse MDL-1/CLEC5A Antibody (226402)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MDL-1/CLEC5A Antibody (226402)

For research use only

Citations for Mouse MDL-1/CLEC5A Antibody (226402)

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