Mouse MUP-1 Antibody (663323)

R&D Systems | Catalog # MAB6560

R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 663323
View all MUP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse MUP-1 synthetic peptide
Accession # NP_112465

Specificity

Detects mouse MUP-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Mouse MUP-1 Antibody (663323)

MUP-1 antibody in NCTC 1469 Mouse Cell Line by Immunocytochemistry (ICC).

MUP‑1 in NCTC 1469 Mouse Cell Line.

MUP-1 was detected in immersion fixed NCTC 1469 mouse liver cell line using Mouse MUP-1 Monoclonal Antibody (Catalog # MAB6560) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse MUP-1 Antibody (663323)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NCTC 1469 mouse liver cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MUP-1

Major urinary protein 1 (MUP-1) is a 20 kDa lipocalin family glycoprotein that is secreted by the liver into the circulation and then excreted in the urine of male mice. Its beta -barrel structure with an internal hydrophobic cavity enables MUP-1 to serve as a carrier for a variety of pheromones. In the circulation, MUP-1 promotes energy expenditure, mitochondrial biogenesis, and oxidative function, and it inhibits hepatic glucose production, triglyceride accumulation, glucose intolerance, and insulin resistance. MUP-1 is downregulated in the liver and circulation of diabetic obese mice.

Long Name

Major Urinary Protein 1

Alternate Names

MUP1

Entrez Gene IDs

17840 (Mouse)

Gene Symbol

MUP1

UniProt

NP_112465

Additional MUP-1 Products

Product Documents for Mouse MUP-1 Antibody (663323)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MUP-1 Antibody (663323)

For research use only

Related Research Areas

Glucose Homeostasis

Citations for Mouse MUP-1 Antibody (663323)

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