Mouse Plexin A1 APC-conjugated Antibody

Name: Mouse Plexin A1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB4309A

Catalog #: FAB4309A Datasheet / COA / SDS

Discontinued Product

FAB4309A has been discontinued.

View all Plexin A1 products.

Detection of Plexin A1 in bEnd.3 Mouse Cell Line by Flow Cytometry.

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Mouse Plexin A1 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10% cross‑reactivity with recombinant mouse (rm) Plexin A3 is observed and no cross-reactivity with rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, or rhPlexin D1 is observed.

Source

Monoclonal Rat IgG_2A Clone # 408305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Plexin A1
Leu49-Gly165
Accession # P70206

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Plexin A1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Plexin A1 APC-conjugated Monoclonal Antibody (Catalog # FAB4309A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Plexin A1

Plexin A1 is an 1894 amino acid, 200 kDa type I transmembrane protein that is a member of the Plexin family of semaphorin signal transducers. Plexin-A1 binds the transmembrane Sema6D directly and binds the Class 3 secreted semaphorins indirectly via neuropilin-1 and 2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. In the region used as an immunogen, mouse and human Plexin A1 share 97% amino acid identity.

Entrez Gene IDs

5361 (Human); 18844 (Mouse)

Alternate Names

NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1