Mouse PRDC/GREM2 Antibody

R&D Systems | Catalog # AF2069

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PRDC/GREM2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PRDC
Arg22-Gln168
Accession # O88273

Specificity

Detects mouse PRDC in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse (rm) Cerberus, rmGremlin, rmDAN, recombinant human (rh) COCO, rhGremlin, and rmBMP-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse PRDC/GREM2 Antibody

Mouse PRDC / GREM2 ELISA Standard Curve

Mouse PRDC / GREM2 ELISA Standard Curve

Recombinant Mouse PRDC/GREM2 (Catalog # 2069-PR) was serially diluted and captured by Goat Anti-Mouse PRDC/GREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2069) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse PRDC/GREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2069) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse PRDC / GREM2 ELISA Standard Curve

Mouse PRDC / GREM2 ELISA Standard Curve

Recombinant Mouse PRDC/GREM2 (Catalog # 2069-PR) was serially diluted and captured by Goat Anti-Mouse PRDC/GREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2069) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse PRDC/GREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2069) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of PRDC/GREM2 by Western Blot

Detection of PRDC/GREM2 by Western Blot

NOG, GREM1, GREM2 have different antagonizing effects on BMP2, TGF-beta 1 and BMP8A. For testing the antagonisms to BMP2, BRE-Luc reporter-containing HEK293T cells were treated with BMP2 (1 nM) and graded doses of NOG (A), GREM1 (B) or GREM2 (C) as indicated. For testing the antagonisms to TGF-beta 1, CAGA-Luc reporter-containing HEK293T cells were treated with TGF-beta 1 (0.3 nM) and graded doses of NOG (E), GREM1 (F) or GREM2 (G) as indicated. For testing the antagonisms to BMP8A, BRE-Luc reporter-containing cells (I–K) or CAGA-Luc reporter-containing cells (M–O) were treated with BMP8A (1 nM) and graded doses of NOG (I and M), GREM1 (J and N) or GREM2 (K and O) as indicated. After treated overnight, reporter activity in the cells was measured. The luciferase data were normalized against the beta -galactosidase levels to correct for transfection efficiency and were expressed as fold changes by normalizing against the values obtained from control cells without treatment. Data are expressed as the mean ± SEM. n = 3. ***P < 0.001. For monitoring the phosphorylated SMAD levels, HEK293T cells were treated with BMP2 (D), TGF-beta 1 (H) or BMP8A (L and P) in the absence or presence of indicated antagonists (1 nM) for 30 min. Cell lysates were subjected to immunoblotting to detect the phosphorylated SMADs. Total forms of SMADs and beta -actin served as loading controls Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36443839), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse PRDC/GREM2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse PRDC (Catalog # 2069-PR)

Mouse PRDC Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse PRDC/GREM2 Biotinylated Antibody (Catalog # BAF2069)
  • Standard: Recombinant Mouse PRDC/GREM2 Protein (Catalog # 2069-PR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PRDC/GREM2

PRDC (protein related to DAN and Cerberus) is a secreted cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, Dan, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot, which form an additional intrasubunit disulfide bond (1, 2). Some members of this family, including PRDC, have an additional cysteine residue used for dimerization (1, 2). Of all the CAN family members, PRDC is most closely related to Gremlin, displaying 52% amino acid sequence identity. PRDC was first identified in a screen for developmentally regulated genes by gene trapping in embryonic stem cells (3). PRDC expression is detected by in situ hybridization in the dorsal edge of the spinal cord at E10.5, in commissural neurons in the caudal part of the spinal cord two days later (3), and in the granulosa cells of selective ovarian follicles (4). In the adult, abundant levels of PRDC are detected by RT-PCR in the mouse ovary, brain, and spleen, and to a lesser degree in the colon, kidney, lung, liver, and uterus (4). PRDC acts as a specific BMP antagonist, binding to and blocking signaling induced by BMP-2 or -4, but not Activin or TGF-beta (4). Thus, PRDC expression in the ovary could be involved in follicular development by antagonizing the inhibitory effects of BMPs on FSH stimulation of progesterone (4).

References

  1. Pearce, J. et al. (1999) Dev. Biol. 209:99.
  2. Avsian-Kretchmer, O. and A. Hsueh (2004) Molecular Endocrinology 18:1.
  3. Minabe-Saegusa, C. et al. (1998) Develop. Growth Differ. 40:343.
  4. Sudo, S. et al. (2004) Jour. Biol. Chem. 279:23134.

Long Name

Protein Related to DAN and Cerberus/Gremlin-2

Alternate Names

CKTSF1B2, DAND3, GREM2

Entrez Gene IDs

64388 (Human); 23893 (Mouse)

Gene Symbol

GREM2

UniProt

O88273

Additional PRDC/GREM2 Products

Product Documents for Mouse PRDC/GREM2 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse PRDC/GREM2 Antibody

For research use only

Related Research Areas

BMP Family

Citations for Mouse PRDC/GREM2 Antibody

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