Mouse/Rat PRAT4B Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5015N Datasheet / COA / SDS

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AF5015N-100UG

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Mouse/Rat PRAT4B Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat PRAT4B in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human PRAT4B and recombinant mouse PRAT4A is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derive recombinant mouse PRAT4B
Thr30-Leu245
Accession # Q8BQ47

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PRAT4B

PRAT4B (protein associated with TLR4-B; also Cnpy4, protein canopy homolog 4) is a member of the canopy family of proteins. It is expressed by diverse cell types, including macrophages, fibroblasts and B cells. PRAT4B appears to be both secreted, and retained internally, where it may stabilize immature TLR protein expression in the ER, and impact its appearance on the cell surface. Mouse PRAT4B is 245 amino acids (aa) in length. It contains a 27 aa signal sequence plus one coiled-coil region (aa 209 - 237). There is one potential splice variant that shows a 13 aa substitution for the C-terminal nine amino acids. Over aa 30 - 245, mouse PRAT4B is 95% and 87% aa identical to rat and human PRAT4B, respectively.

Long Name

Protein Associated with Toll-like Receptor 4B

Entrez Gene IDs

245812 (Human); 66455 (Mouse); 363886 (Rat)

Alternate Names

canopy 4 homolog (zebrafish); CNPY4; MGC40499PRAT4BPRotein Associated with Tlr4; PRAT4B; protein canopy homolog 4