Mouse ROBO4 APC-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: ROBO4

ROBO4, also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family. ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration. Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium. Expression in early hematopoietic progenitors is also reported. Vascular endothelial ROBO4 is expressed at highest levels in during development and vascular remodeling, including tumor angiogenesis. It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF‑induced vascular leak. ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers. ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation. Effects on cell movement may be mediated through intracellular binding of WASP-, Ras/Rac/Rho-, Mena-, Src-, or Paxillin‑related proteins, all of which affect the cytoskeleton. Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis. 

Product Datasheets

Related Research Areas

• Netrins and Receptors
• Slit Ligands and ROBO Receptors