Mouse Siglec-H APC-conjugated Antibody

Name: Mouse Siglec-H APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB7319A

Catalog #: FAB7319A Datasheet / COA / SDS

Discontinued Product

FAB7319A has been discontinued.

View all Siglec-H products.

Detection of Siglec‑H in Mouse Splenocytes by Flow Cytometry.

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All Siglec-H Antibodies

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Mouse Siglec-H APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Siglec-H.

Source

Monoclonal Rat IgG₁ Clone # 730407

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Siglec-H
Met1-Gly269
Accession # NP_848821

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec‑H in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse Siglec-H APC-conjugated Monoclonal Antibody (Catalog # FAB7319A) or (B) Rat IgG₁Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-H

Siglec-H, a murine CD33-related Siglec-like molecule and a member of the Sialic Acid-binding Immunoglobulin (Ig)-like Lectin (Siglec) family, is expressed specifically on Plasmacytoid Dendritic Cell (pDC) precursors in the bone marrow, spleen, blood, and lymph nodes.

Long Name

Sialic Acid Binding Ig-like Lectin 7

Entrez Gene IDs

233274 (Mouse)

Alternate Names

6430529G09Rik; Siglech sialic acid binding Ig-like lectin H; SiglecH; Siglec-H