Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-reported

Cited:

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG2 kappa Clone # GL-3
View all TCR gamma/delta Primary Antibodies »

Product Specifications

Immunogen

Mouse intraepithelial lymphocytes

Specificity

Detects mouse TCR gamma / delta.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG2 kappa

Scientific Data Images for Mouse TCR gamma/delta Antibody (GL-3)

Detection of TCR gamma / delta antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of TCR gamma / delta in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (FAB4841A) and either (A) Hamster Anti-Mouse TCR gamma / delta Monoclonal Antibody (Catalog # MAB7297) or (B) Normal Hamster IgG Control Antibody followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody.

Applications for Mouse TCR gamma/delta Antibody (GL-3)

Application
Recommended Usage

CyTOF-reported

Spitzer, M. et al. (2015) Science 349: 1259425.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCR gamma/delta

The gamma delta T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3 delta glycoprotein subunits of the Ig superfamily. T gamma delta cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3 delta are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3 delta share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3 delta. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma delta T cells, were used as the immunogen for the GL-3/5E11 antibody(1).

References

  1. Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.

Alternate Names

delta polypeptide, T3D, TiT3

Entrez Gene IDs

915 (Human); 12500 (Mouse); 25710 (Rat)

Gene Symbol

CD3D

Additional TCR gamma/delta Products

Product Documents for Mouse TCR gamma/delta Antibody (GL-3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TCR gamma/delta Antibody (GL-3)

For research use only

Citations for Mouse TCR gamma/delta Antibody (GL-3)

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