Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 418601
View all TLR6 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with mouse TLR6
Phe39-Thr806
Accession # BAA78632

Specificity

Detects mouse TLR6. Stains mouse TLR6 transfectants and not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse TLR6 Antibody

Detection of TLR6 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of TLR6 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/ macrophage cell line was stained with Rat Anti-Mouse TLR6 Monoclonal Antibody (Catalog # MAB1533, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Applications for Mouse TLR6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB1533 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR6

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).

References

  1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  2. Roeder, A. et al. (2004) Med. Mycol. 42:485.
  3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  4. Wetzler, L.M. (2003) Vaccine 21:S55.
  5. Takeuchi, O. et al. (1999) Gene 231:59.
  6. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
  7. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
  8. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
  9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
  10. Bulut, Y. et al. (2001) J. Immunol. 167:987.
  11. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
  12. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
  13. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
  14. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.
  15. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.

Long Name

Toll-like Receptor 6

Alternate Names

CD286

Entrez Gene IDs

10333 (Human); 21899 (Mouse); 305353 (Rat)

Gene Symbol

TLR6

UniProt

BAA78632

Additional TLR6 Products

Product Documents for Mouse TLR6 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse TLR6 Antibody

For research use only

Citations for Mouse TLR6 Antibody

Customer Reviews for Mouse TLR6 Antibody (2)

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  • Mouse TLR6 Antibody
    Name: Celine Beamer
    Application: Flow Cytometry
    Sample Tested: Bone marrow-derived macrophages and alveolar macrophages
    Species: Mouse
    Verified Customer | Posted 03/18/2016
    Acute silica exposure reduced TLR 6 expression on F4-80+CD11c+ alveolar macrophages. C57Bl/6 wild-type mice were exposed to saline (25 μl, black line) or silica (1 mg, gray line) through intranasal aspiration. After 4 h, whole lungs were lavaged, cells immunostained, and surface markers assessed by flow symmetry using a BD FACS Aria II. Data shown as histograms relative to unstained control (dashed line).
    Mouse TLR6 Antibody MAB1533
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22633994
    Species: Mouse
    Verified Customer | Posted 02/10/2015

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