Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Immunocytochemistry

Cited:

Western Blot, Flow Cytometry, Mass Spectrometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 109646
View all Tyro3/Dtk Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tyro3/Dtk
Ala31-Ser418
Accession # P55144

Specificity

Detects mouse Tyro3/Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tyro3/Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Tyro3/Dtk Antibody (109646)

Detection of Tyro3/Dtk in Transfected HEK 293 (positive) and mIMCD-3 (positive).

Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and mIMCD‑3 mouse epithelial cell line (positive) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Tyro3/Dtk in Transfected HEK 293 (positive) and wild-type HEK 293 (negative) cells.

Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and absent in wild-type HEK293 human embryonic kidney cells (negative) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse Dtk/TYRO3 by Western Blot

Detection of Mouse Dtk/TYRO3 by Western Blot

Expression of TAM RTKs, Gas6 and Protein S.(A) Western blot analyses of the liver lysates for the examination of TAM RTKs, Gas6 and Protein S. (B) Expression of TAM RTKs, Gas6 and Protein S in isolated liver cells: parenchymal cells (PCs), Kupffer cells(KCs) and sinusoidal endothelial cells (SECs). The primary cells were subjected to Western blotting. (C) Immunohistochemistry for the detection of TAM RTKs, Gas6 and Protein S. Arrowheads indicate PCs, and arrows indicate spindle-shaped sinusoidal cells corresponding to KCs and SECs. In negative controls (islets), sections were incubated with primary antibodies pre-incubated with an excess of blocking peptide. The livers of 15-week-old WT mice were used for the protein analyses. The images are representatives of at least three experiments. Scale bar = 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23799121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Dtk/TYRO3 by Western Blot

Detection of Mouse Dtk/TYRO3 by Western Blot

Expression of TAM RTKs, Gas6 and Protein S.(A) Western blot analyses of the liver lysates for the examination of TAM RTKs, Gas6 and Protein S. (B) Expression of TAM RTKs, Gas6 and Protein S in isolated liver cells: parenchymal cells (PCs), Kupffer cells(KCs) and sinusoidal endothelial cells (SECs). The primary cells were subjected to Western blotting. (C) Immunohistochemistry for the detection of TAM RTKs, Gas6 and Protein S. Arrowheads indicate PCs, and arrows indicate spindle-shaped sinusoidal cells corresponding to KCs and SECs. In negative controls (islets), sections were incubated with primary antibodies pre-incubated with an excess of blocking peptide. The livers of 15-week-old WT mice were used for the protein analyses. The images are representatives of at least three experiments. Scale bar = 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23799121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mouse Tyro3 / Dtk ELISA Standard Curve

Mouse Tyro3 / Dtk ELISA Standard Curve

Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT) was serially diluted and captured by Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF759) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse Tyro3/Dtk Antibody (109646)

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed Transfected HEK293 human embryonic kidney cells (positive), mIMCD‑3 mouse epithelial cells (positive), and wild-type HEK293 human embryonic kidney cells (negative)

Western Blot

1 µg/mL
Sample: Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT)

Mouse Tyro3/Dtk Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: (Catalog # BAF759)
  • Standard: Recombinant Mouse Tyro3/Dtk Fc Chimera Protein, CF (Catalog # 759-DT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

P55144

Additional Tyro3/Dtk Products

Product Documents for Mouse Tyro3/Dtk Antibody (109646)

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Product Specific Notices for Mouse Tyro3/Dtk Antibody (109646)

For research use only

Citations for Mouse Tyro3/Dtk Antibody (109646)

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Protocols

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