MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free

Novus Biologicals | Catalog # H00009252-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 2B11

Format

Azide and BSA Free
View all MSK1 Primary Antibodies »

Product Specifications

Immunogen

RPS6KA5 (AAH17187.1, 271 a.a. ~ 370 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ILKSEPPYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSEK

Specificity

RPS6KA5 - ribosomal protein S6 kinase, 90kDa, polypeptide 5 (2B11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Scientific Data Images for MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free

Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - Analysis of RPS6KA5 expression in transfected 293T cell line by RPS6KA5 monoclonal antibody (M01), clone 2B11.Lane 1: RPS6KA5 transfected lysate(89.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Immunocytochemistry/ Immunofluorescence: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - Analysis of monoclonal antibody to RPS6KA5 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - RPS6KA5 monoclonal antibody (M01), clone 2B11. Western Blot analysis of RPS6KA5 expression in HeLa ( Cat # L013V1 ).
Proximity Ligation Assay: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Proximity Ligation Assay: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01]

Proximity Ligation Assay: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - Analysis of protein-protein interactions between NR4A1 and RPS6KA5. HeLa cells were stained with anti-NR4A1 rabbit purified polyclonal 1:1200 and anti-RPS6KA5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MSK1

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Long Name

Mitogen- and Stress-activated Protein Kinase 1

Alternate Names

RPS6KA5

Entrez Gene IDs

9252 (Human)

Gene Symbol

RPS6KA5

UniProt

AAH17187 (Human)

Additional MSK1 Products

Product Documents for MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail