Myosin heavy chain 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94624

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Myosin heavy chain 2/MYH2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Myosin heavy chain 2 (NP_001093582.1). AQLFSGAQTAEGEGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

223 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Myosin heavy chain 2 Antibody - BSA Free

Western Blot: Myosin heavy chain 2 AntibodyAzide and BSA Free [NBP2-94624]

Western Blot: Myosin heavy chain 2 AntibodyAzide and BSA Free [NBP2-94624]

Western Blot: Myosin heavy chain 2 Antibody [NBP2-94624] - Analysis of extracts of Mouse skeletal muscle cells, using MYH2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: Myosin heavy chain 2 AntibodyAzide and BSA Free [NBP2-94624]

Western Blot: Myosin heavy chain 2 AntibodyAzide and BSA Free [NBP2-94624]

Western Blot: Myosin heavy chain 2 Antibody [NBP2-94624] - Analysis of extracts of Rat skeletal muscle cells, using MYH2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.
Myosin heavy chain 2 Antibody

Myosin heavy chain 2 Antibody [NBP2-94624] -Analysis of mouse skeletal muscle cells using MYH2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Myosin heavy chain 2 Antibody [NBP2-94624] -Analysis of mouse skeletal muscle cells using MYH2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Myosin heavy chain 2 Antibody

Myosin heavy chain 2 Antibody [NBP2-94624] -Analysis of rat skeletal muscle cells using MYH2 Rabbit pAb (A15292) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Myosin heavy chain 2 Antibody [NBP2-94624] -Analysis of rat skeletal muscle cells using MYH2 Rabbit pAb (A15292) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Myosin heavy chain 2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] -

Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] - Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using Myosin heavy chain 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Myosin heavy chain 2 Antibody - Azide and BSA Free

Western Blot: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] -

Western Blot: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] - Western blot analysis of lysates from U-2 OS cells using Myosin heavy chain 2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 60 s.
Myosin heavy chain 2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] -

Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] - Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Myosin heavy chain 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Myosin heavy chain 2 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Myosin heavy chain 2/MYH2

Myosin II is the so called "classical" myosin, being the first type of myosin to be discovered. Myosin II forms bipolar filaments that interact with actin filaments to produce contraction. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. The myosin II molecule is composed of two heavy chains and two sets of light chains. The light chains are the Essential Light Chains (ELC) and Regulatory Light Chains (RLC). Overall activity and polymerization can be modulated by phosphorylation on the light chains and the C terminus of the heavy chains respectively.

Long Name

Myosin Heavy Chain 2

Alternate Names

IBM3, MYH2, MyHC-2a, MyHC-IIa, MYHSA2

Gene Symbol

MYH2

Additional Myosin heavy chain 2/MYH2 Products

Product Documents for Myosin heavy chain 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Myosin heavy chain 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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