N-Acetylcysteine

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Lung Organoids. Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), SB 202190 (Catalog # 1264), Nicotinamide (Catalog # 4106), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various lung cell types. Lung organoids were stained with (A) anti-Cytokeratin 5 (KRT5) (green) and with anti-p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (B) Anti-Podoplanin (PDPN) (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and anti- p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (C, D) Anti-MUC5AC (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and anti-SOX2 (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Liver Organoids. Adult stem cell-derived liver organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), A 83-01 (Catalog # 2939), Forskolin (Catalog # 1099), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes N-Acetylcysteine, HEPES (Catalog # 3173), Nicotinamide (Catalog # 4106), A 83-01 (Catalog # 2939), along with other reagents. Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

Application of N-Acetylcysteine in Culture and Characterization of iPSC-derived Human Intestinal Organoids. iPSC-derived human intestinal organoids were cultured using N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Human/Mouse/Rat Vimentin Antibody (Catalog # MAB2105; green) and a Human/Mouse Desmin Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Human/Mouse E-Cadherin Antibody (Catalog # AF748; green), a Human MUC2 Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).

Application of N-Acetylcysteine in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids. Adult stem cells isolated from human descending colon were embedded and then cultured for 30 days in intestinal organoid culture medium, which includes N-Acetylcysteine, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), A 83-01 (Catalog # 2939), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with Anti-MUC2 (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained for E-Cadherin (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue). (B) Organoids were fixed and stained with Human Chromogranin A Antibody(Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained for E-Cadherin (Catalog # AF3844; red) and DAPI (Catalog # 5748; blue).