This protocol outlines a method for culturing normal human lung organoids based on protocols described by Norman Sachs and Hans Clevers (1). The procedure that is described allows for the long-term expansion of airway organoids in vitro from adult human lung tissue. Starting with a suspension of isolated and dissociated human lung tissue, the reagents and steps necessary to generate and passage human lung organoids are fully detailed, allowing for the generation of an in vitro lung model that can be used for pulmonary disease modeling and drug toxicity studies. This lung organoid protocol utilizes reagents across the Bio-Techne portfolio including recombinant proteins, small molecules, media supplements, and Cultrex® Organoid Qualified Basement Membrane Extract (BME) as a scaffold.
The protocol provides some specific tips and recommendations for culturing human lung organoids including:
- Recommended media for expanding and differentiating lung organoids
- Considerations and tips for starting lung organoid cultures
- Methods to optimize Cultrex Organoid Qualified BME as a matrix scaffold
- Tips for successful passaging and cryopreservation
- Data examples for lung organoid characterization
- Sachs, N. et al. (2019) EMBO J. 34:e100300.
Optimizing Organoid Culture Conditions: The Importance of Growth Factor Bioactivity and Reagent Consistency Application Note
View this application note to learn about the importance of protein bioactivity and reagent consistency for optimizing organoid culture conditions. See how R&D Systems products can help researchers be confident that their culture conditions are consistent and reproducible from one experiment to the next.
View the different formats of Cultrex Basement Membrane Extract (BME) for 2-D and 3-D cell culture that we offer to support your application-specific needs.
Explore our wide selection of proteins for culturing and expanding human lung organoids. From FGFs to Noggin and R-Spondin 1, we have the most trusted, most cited recombinant proteins you need to successfully generate and passage human lung organoids.
We offer a wide range of Tocris® small molecules that are commonly used as 3-D growth matrix components for generating different types of organoids and sustaining long-term growth.
Our portfolio includes media supplements for use in a variety of cell culture systems. We provide general use media supplements, supplements for specialized cell culture, and protein-free media for the cryopreservation of cells.
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