N4BP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76531

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all N4BP1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1.

Specificity

At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N4BP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531]

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531]

Immunocytochemistry/Immunofluorescence: N4BP1 Antibody [NBP1-76531] - Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 ug/mL.
N4BP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531] -

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531] - Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 ug/mL.
N4BP1 Antibody - BSA Free

Western Blot: N4BP1 Antibody - BSA Free [NBP1-76531] -

Western Blot: N4BP1 Antibody - BSA Free [NBP1-76531] - WB Validation in Human Cell LinesLoading: 15 ug of lysates Antibodies: N4BP1, 1 ug/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 sec

Applications for N4BP1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: N4BP1

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Alternate Names

FLJ31821, KIAA0615, MGC176730, NEDD4 binding protein 1, NEDD4-binding protein 1

Gene Symbol

N4BP1

UniProt

NP_694574 (Human)

Additional N4BP1 Products

Product Documents for N4BP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for N4BP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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