NARF Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55660

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NARF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KTDDQENVSADAPSPAQENGEKGEFHKLADAKIFLSDCLACDSCMTAEEGVQLSQQNAKDFFRVLNLNKKCDTSKHKVLVVSVCPQSLPYFAAKFNLSVTDASRRLCGFLKSLG

Reactivity Notes

Mouse 84%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NARF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NARF Antibody [NBP2-55660]

Immunocytochemistry/ Immunofluorescence: NARF Antibody [NBP2-55660]

Immunocytochemistry/Immunofluorescence: NARF Antibody [NBP2-55660] - Human MCF7 cells was stained with primary NARF antibody (1:50), followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody [Texas Red] [NB120-7088] (1:1000). Image added from verified customer review.
Immunocytochemistry/ Immunofluorescence: NARF Antibody [NBP2-55660]

Immunocytochemistry/ Immunofluorescence: NARF Antibody [NBP2-55660]

Immunocytochemistry/Immunofluorescence: NARF Antibody [NBP2-55660] - ICC/IF of human cell line MCF7 shows localization to nucleus & nucleoli. Antibody staining is in green.
Immunocytochemistry/ Immunofluorescence: NARF Antibody [NBP2-55660]

Immunocytochemistry/ Immunofluorescence: NARF Antibody [NBP2-55660]

Immunocytochemistry/Immunofluorescence: NARF Antibody [NBP2-55660] - SUM-159PT human breast cancer cell line. Antibody was used at 1:50 for labeling and microscopy analysis of the distribution of NARF proteins in SUM-159PT breast cancer cells. ICC/IF image submitted by a verified customer review.
Knockdown Validated: NARF Antibody [NBP2-55660]

Western Blot: NARF Antibody [NBP2-55660]

Western Blot: NARF Antibody [NBP2-55660] - Cell lysate from MCF7 cells grown in DMEM medium. 30 ug cell lysate per lane. 10% SDS-PAGE. NARF antibody at 1:1000 dilution. HRP-conjugated secondary donkey anti-rabbit IgG polyclonal antibody at 1:2000 dilution. WB image submitted by a verified customer review.
NARF Antibody

Western Blot: NARF Antibody [NBP2-55660] -

Western Blot: NARF Antibody [NBP2-55660] - Analysis in human cell line MOLT-4.

Applications for NARF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Knockdown Validated

Validated from a verified customer review

Western Blot

0.04 - 0.4 ug/mL
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-55660 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NARF

NARF (Nuclear Prelamin A Recognition Factor) is involved in electron transport, heterochromatin organization and regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor. NARF is known to have interactions with LMNA, LMNB1, PCB4, QKI and TAF9. NARF has been studied in relation to glaucoma.

Alternate Names

FLJ10067, IOP2DKFZp434G0420, Iron-only hydrogenase-like protein 2, nuclear prelamin A recognition factor, prenyl-dependent prelamin A binding protein

Gene Symbol

NARF

Additional NARF Products

Product Documents for NARF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NARF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NARF Antibody - BSA Free

Customer Reviews for NARF Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 的 2 reviews Showing All
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  • NARF Antibody
    Name: Yongkang Yang
    Application: Immunofluorescence
    Sample Tested: SUM-159PT human breast cancer cell line
    Species: Human
    Verified Customer | Posted 03/17/2021
    Immunofluorescence: NARF Antibody [NBP2-55660, 1:50 dilution] was used for labeling and microscopy analysis of the distribution of NARF proteins in SUM-159PT breast cancer cells.
    NARF Antibody - BSA Free NBP2-55660
  • NARF Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: Cell lysate from MCF7 cells grown in DMEM medium
    Species: Human
    Verified Customer | Posted 08/16/2019
    30ug MCF-7 cell lysate/lane, 10% SDS-PAGE; Primary antibody (NARF Antibody (NBP2-55660), Lot: R70828): 1:1000 diluted in 5% milk incubate at 4℃ for overnight; Secondary antibody: NA934V, Donkey Anti-Rabbit IgG Polyclonal Antibody (HRP (Horseradish Peroxidase)-  GE Healthcare (Lot: 16908227) (1:2000 diluted in 5% milk). Incubate at room temperature for 1 hour.
    NARF Antibody - BSA Free NBP2-55660

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Protocols

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FAQs

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