NEK1 Antibody

Novus Biologicals | Catalog # NBP3-48625

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NEK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NEK1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK1 Antibody

NEK1 Antibody

NEK1 Antibody-NBP3-48625

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000.
NEK1 Antibody

NEK1 Antibody-NBP3-48625

U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nek1 protein stained by Nek1 antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 μm.
NEK1 Antibody

NEK1 Antibody-NBP3-48625

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
NEK1 Antibody

NEK1 Antibody-NBP3-48625

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody diluted at 1:1000.
NEK1 Antibody

Western Blot: NEK1 Antibody [NBP3-48625] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NEK1 antibody (NBP3-48625) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for NEK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEK1

Belonging to the NEK Ser/Thr protein kinase family, NEK1 plays an important role in the cell by responding to DNA damage. Molecular functions for NEK1 include protein serine/threonine kinase activity, protein tyrosine kinase activity, protein binding and ATP binding. More specifically, NEK1 is involved in cell cycle regulation, and the protein encoded by this gene is a serine/threonine kinase which is found in a centrosomal complex with FEZ1. Additionally, NEK1 also appears to possess tyrosine kinase activity and is involved in the control of meiosis and cilium assembly. NEK1 is known to interact with FEZ1, FEZ2, KIF3A, TSC2 and MRE11A. Common diseases for NEK1 include adenocarcinoma, cancer, short rib-polydactyly syndrome II and epithelial neoplasia.

Alternate Names

DKFZp686D06121, DKFZp686K12169, EC 2.7.11, EC 2.7.11.1, KIAA1901MGC138800, Never in mitosis A-related kinase 1, NIMA (never in mitosis gene a)-related kinase 1, NimA-related protein kinase 1, NY-REN-55, protein-serine/threonine kinase, Renal carcinoma antigen NY-REN-55, serine/threonine-protein kinase Nek1, SRPS2

Gene Symbol

NEK1

Additional NEK1 Products

Product Documents for NEK1 Antibody

Certificate of Analysis

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Product Specific Notices for NEK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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