NELF-E Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55487

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NELF-E Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: VYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NELF-E Antibody - BSA Free

Western Blot: NELF-E Antibody [NBP2-55487]

Western Blot: NELF-E Antibody [NBP2-55487]

Western Blot: NELF-E Antibody [NBP2-55487] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: NELF-E Antibody [NBP2-55487]

Immunocytochemistry/ Immunofluorescence: NELF-E Antibody [NBP2-55487]

Immunocytochemistry/Immunofluorescence: NELF-E Antibody [NBP2-55487] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
Western Blot: NELF-E Antibody [NBP2-55487]

Western Blot: NELF-E Antibody [NBP2-55487]

Western Blot: NELF-E Antibody [NBP2-55487] - Analysis using Anti-NELFE antibody NBP2-55487 (A) shows similar pattern to independent antibody NBP1-88036 (B).
NELF-E Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: NELF-E Antibody - BSA Free [NBP2-55487]

Chromatin Immunoprecipitation-exo-Seq: NELF-E Antibody - BSA Free [NBP2-55487]

ChIP-Exo-Seq composite graph for Anti-NELFE (NBP2-55487) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for NELF-E Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NELF-E

NELF-E is encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq]

Alternate Names

D6S45, negative elongation factor E, negative elongation factor polypeptide E, NELFE, NELF-ERDmajor histocompatibility complex gene RD, nuclear protein, RD RNA binding protein, RD RNA-binding protein, RDP, RNA-binding protein RD

Gene Symbol

NELFE

Additional NELF-E Products

Product Documents for NELF-E Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NELF-E Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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