NHP XL Cytokine Luminex® Performance Premixed Kit

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NHP XL Cytokine Luminex® Performance Premixed Kit Summary

Panel Luminex Performance NHP XL Cytokine Panel
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Primate
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

View Luminex High Performance Non-human Primate XL validation data on rndsystems.com.

Make your selections below to configure your assay

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Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split
Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Kit Components
  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**
  • High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.


Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 31 different serum assays.

  n=40 (20x2 levels) n=31
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
BDNF 8.16 13.3
CCL2/MCP-1 3.02 10.5
CCL5/RANTES 3.72 17.0
CCL11/Eotaxin 7.98 15.4
CCL20/MIP-3 alpha 8.41 17.3
CD40 Ligand 9.30 15.0
CXCL2/GRO beta 7.76 13.1
CXCL10/IP-10 2.95 12.2
CXCL11/I-TAC 6.23 13.7
CXCL13/BLC 5.79 12.5
FGF basic 5.60 13.1
G-CSF 5.55 14.2
GM-CSF 7.11 14.1
Granzyme B 9.76 18.6
IFN-alpha 5.17 12.4
IFN-beta 10.9 15.2
IFN-gamma 6.36 13.0
IL-1 beta 2.55 12.7
IL-10 8.55 14.1
IL-12 p70 4.92 17.7
IL-13 7.97 17.5
IL-15 4.99 18.2
IL-17A 4.38 19.0
IL-2 5.32 18.1
IL-21 5.84 19.0
IL-4 4.92 17.5
IL-5 4.14 16.5
IL-6 6.80 17.8
IL-7 6.57 17.7
IL-8/CXCL8 6.87 17.5
PDGF-AA 6.52 25.0
PDGF-BB 3.37 16.9
PD-L1 8.36 19.4
TGF-alpha 5.43 18.7
TNF-alpha 3.68 17.2
VEGF 4.79 18.4


Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.

  Linearity Spiked
Analyte Average % Average %
CCL2/MCP-1 75 135
CCL11/Eotaxin 93 138
CCL20/MIP-3 alpha 105 111
CD40 Ligand 73 105
CXCL2/GRO beta 95 81
CXCL10/IP-10 78 72
FGF basic NV
Granzyme B NO 122
IFN-alpha NO 118
IFN-beta NO 71
IFN-gamma NO 124
IL-1 beta NO 68
IL-10 NO 104
IL-12 p70 NO 109
IL-13 NO 118
IL-15 NO 90
IL-17A NO 90
IL-2 NO 89
IL-21 NO 121
IL-4 NO 80
IL-5 NO 95
IL-6 NO 84
IL-7 NO 97
IL-8/CXCL8 121 HE
PDGF-AA 101 99
PD-L1 80 HE
TGF-alpha NV
TNF-alpha NO 85
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

NHP Luminex Standard Curves (Cell Culture Supernates)

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for NHP XL Cytokine Luminex® Performance Premixed Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Recall responses in the lung environment are impacted by age in a pilot study of Mycobacterium bovis-BCG vaccinated rhesus macaques
    Authors: JM Scordo, TJ Piergallin, AM Olmo-Fontá, A Thomas, HP Raué, M Slifka, J Turner
    Experimental gerontology, 2022-07-30;167(0):111904.
    Species: Rhesus Macaque
    Sample Types: Cell Culture Supernates
  2. Liver-directed lentiviral gene therapy corrects hemophilia A mice and achieves normal-range factor VIII activity in non-human primates
    Authors: M Milani, C Canepari, T Liu, M Biffi, F Russo, T Plati, R Curto, S Patarroyo-, D Drager, I Visigalli, C Brombin, P Albertini, A Follenzi, E Ayuso, C Mueller, A Annoni, L Naldini, A Cantore
    Nature Communications, 2022-05-04;13(1):2454.
    Species: Macaca leonina
    Sample Types: Serum


  1. Which primate species have been tested with the NHP XL Cytokine Premixed Kit?

    • The kit has been tested and validated with Cynomolgus monkey and Rhesus macaque samples. The kit is broadly designated as reactive with non-human primate samples due to the expected high homology across primate species.

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Reviews for NHP XL Cytokine Luminex® Performance Premixed Kit

Average Rating: 4.7 (Based on 3 Reviews)

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By Anonymous on 06/15/2022
Sample Tested: Blood mononuclear cells (PBMCs)

NHP XL Cytokine Luminex Performance Premixed Kit
By Lu Bai on 05/06/2022
Sample Tested: Non human primary

NHP XL Cytokine Luminex Performance Premixed Kit
By Anonymous on 10/16/2020
Sample Tested: Plasma

This kit works well with Drop Array plates