High Performance Luminex Assays: R&D Systems

Luminex High Performance Assays are our most accurate and precise bead-based multianalyte Luminex assay kits. The assays rely on panel-optimized diluents that provide maximum performance for up to 46 analytes, depending on the panel selected. Analytes can be ordered either as a complete panel or as a smaller subset from any of the panels listed below. The kits can be used to measure target analytes in their validated sample types, which may include cell culture supernates, serum, plasma, urine, saliva, and milk.

Build by Analyte

Features

All analytes in a given panel are optimized and tested together to ensure maximum performance for the entire panel
Kits undergo similar validation testing as the Quantikine single analyte assays and are correlated with Quantikine ELISAs, when available
Kits consist of base kits and bead sets, which can be ordered individually or as premixed kits
All assays can be performed in 2.5-5 hours
Assays require a small sample volume (<50 µL)

Available Panels

High Performance - Fixed Luminex Assay Panels	High Performance - Configurable Luminex Assay Panels
Human Cytokine 15-plex	Human Biomarker Panel A
Human Chemokine 8-plex	Human Cytokine Panel A
Human Growth Factor 21-plex	Human High Sensitivity Cytokine Panel A
Human Immunotherapy 25-plex	Human High Sensitivity Cytokine Panel B
Human Th1/Th2 13-plex	Human Immuno-oncology Panel
Human Th9/Th17/Th22 18-plex	Human Kidney Biomarker Panel
Human TIMP 3-plex	Human Metabolic Panel
Human XL Cytokine 46-plex	Human MMP Panel
Mouse XL Cytokine Assay 45-plex	Human Obesity Panel
	Human Tumor Biomarker Panel
	Human XL Cytokine Panel
	Mouse XL Cytokine Assay Panel
	Multi-species TGF-beta Panel
	NHP XL Cytokine Panel

Choosing Between High Performance & Discovery Luminex Assays

	Luminex Discovery Assays	Luminex “Premixed” High Performance Assays	Luminex “User Mixed” High Performance Assays	Luminex Fixed High Performance Assays
Maximum Analyte Multiplex	>50	46	46	46
Sample Volume (μL)	25-50	25-50	25-50	25-50
Microparticles Premixed	Yes	Yes	No	Yes
Biotin Antibodies Premixed	Yes	Yes	No	Yes
Controls Available	No	Yes	Yes	Yes
Premix QC Prior to Shipping	Yes, full QC	Yes, Confirmational QC	Yes, Confirmational QC	Yes, Confirmational QC
# Analytes Available	>450	>100	>100	>100
Species Available	Human, Mouse, Rat, Porcine	Human, Mouse, Primate	Human, Mouse, Primate	Human, Mouse
Validated Sample Types	Cell culture supernatants, serum, EDTA plasma, Heparin plasma. For mouse analytes only, tissue lysates.	Cell culture supernatants, serum, EDTA plasma, Heparin plasma; For select panels: saliva, urine, milk.	Cell culture supernatants, serum, EDTA plasma, Heparin plasma; for select panels: saliva, urine, milk.	Same as "Premixed"
Analyte Selection	User defined	Predetermined but customizable	Predetermined but customizable	Fixed
Assay Format	All required components included	All required components included	Bead sets and base kits separate	All required components included
Delivery Time	U.S. orders ship in 7 business days.	U.S. orders ship in 5 business days.	U.S. orders ship in 2 days.	U.S. orders usually ship in 1 day.
# Panels Available	Custom	10 customizable panels, including 2 high sensitivity panels.	13 customizable panels, including 2 high sensitivity panels.	9 fixed panels.

Luminex High Performance Assays Correlate to Gold Standard Quantikine ELISA Kits

Cell culture supernate from adipocytes were analyzed using both Luminex High Performance Assays and Quantikine ELISAs. 

Cell culture supernate from pre-adipocytes before and after differentiation were harvested and frozen at the time points indicated.  Following collection, samples were analyzed using Human Leptin and Human Total MMP-3 Quantikine ELISAs (blue bars; Catalog # DLP00 and DMP300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LBHS000 and LMPM000, respectively).

Cell culture supernate from adipocytes analyzed using both Luminex High Performance Assays and Quantikine ELISAs

Cell culture supernate from SW1353 human chondrosarcoma cells were analyzed using both Luminex High Performance Assays and Quantikine ELISAs.

Cell culture supernate from SW1353 cells were either unstimulated or stimulated with 5 ng/mL IL-1 alpha and 10 ng/mL OSM, harvested 24 hours later, and frozen.  Following collection, samples were analyzed using Human CXCL10/IP-10, Human Total MMP-3, and Human Pro-MMP-13 Quantikine ELISAs (blue bars; Catalog # DIP100, DMP300, and DM1300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LBHS000 and LMPM000, respectively).