Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5F4

Format

BSA Free
View all NM23-H1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human NM23-H1 (1-152aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Novus Biologicals Mouse NM23-H1 Antibody (5F4) - BSA Free (NBP2-59498) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NM23-H1 Antibody (5F4) - BSA Free

Western Blot: NM23-H1 Antibody (5F4)BSA Free [NBP2-59498]

Western Blot: NM23-H1 Antibody (5F4)BSA Free [NBP2-59498]

Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC3 cell lysate
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (5F4) - BSA Free [NBP2-59498]

Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (5F4) - BSA Free [NBP2-59498]

Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (5F4) [NBP2-59498] - Analysis of NME1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of NME1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: NM23-H1 Antibody (5F4)BSA Free [NBP2-59498]

Western Blot: NM23-H1 Antibody (5F4)BSA Free [NBP2-59498]

Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - Lane 1: NME1, Lane 2: NME2 Lane 3: NME3 Lane 4: NME4

Applications for NM23-H1 Antibody (5F4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NM23-H1

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Long Name

Non-metastatic Protein 23 Homolog 1

Alternate Names

GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1

Gene Symbol

NME1

Additional NM23-H1 Products

Product Documents for NM23-H1 Antibody (5F4) - BSA Free

Certificate of Analysis

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Product Specific Notices for NM23-H1 Antibody (5F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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