pan Cadherin Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13122

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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cadherin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human pan Cadherin. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for pan Cadherin Antibody - BSA Free

Western Blot: pan Cadherin Antibody [NBP3-13122]

Western Blot: pan Cadherin Antibody [NBP3-13122]

Western Blot: pan Cadherin Antibody [NBP3-13122] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with pan Cadherin antibody (NBP3-13122) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: pan Cadherin Antibody [NBP3-13122]

Immunocytochemistry/ Immunofluorescence: pan Cadherin Antibody [NBP3-13122]

Immunocytochemistry/Immunofluorescence: pan Cadherin Antibody [NBP3-13122] - pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: pan Cadherin stained by pan Cadherin antibody (NBP3-13122) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Western Blot: pan Cadherin Antibody [NBP3-13122]

Western Blot: pan Cadherin Antibody [NBP3-13122]

Western Blot: pan Cadherin Antibody [NBP3-13122] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody (NBP3-13122) diluted at 1:5000.
Western Blot: pan Cadherin Antibody [NBP3-13122]

Western Blot: pan Cadherin Antibody [NBP3-13122]

Western Blot: pan Cadherin Antibody [NBP3-13122] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody (NBP3-13122) diluted at 1:4000.
pan Cadherin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: pan Cadherin Antibody [NBP3-13122] -

pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in ice-cold MeOH for 5 min.
Green: pan Cadherin protein stained by pan Cadherin antibody (NBP3-13122) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for pan Cadherin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cadherin

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Long Name

Cadherin Pan Specific

Alternate Names

Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDHE, cell-CAM 120/80, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVOE-Cadherin, uvomorulin

Gene Symbol

CDH1

Additional Cadherin Products

Product Documents for pan Cadherin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for pan Cadherin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

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Protocols

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FAQs

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