PBK Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12989

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (93%), Chimpanzee (99%), Mouse (90%), Porcine (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PBK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PBK. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PBK Antibody - BSA Free

Western Blot: PBK Antibody [NBP3-12989]

Western Blot: PBK Antibody [NBP3-12989]

Western Blot: PBK Antibody [NBP3-12989] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:60000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: PBK Antibody [NBP3-12989]

Western Blot: PBK Antibody [NBP3-12989]

Western Blot: PBK Antibody [NBP3-12989] - PBK antibody detects PBK protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:1000.
Western Blot: PBK Antibody [NBP3-12989]

Western Blot: PBK Antibody [NBP3-12989]

Western Blot: PBK Antibody [NBP3-12989] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:1000.
Immunoprecipitation: PBK Antibody [NBP3-12989]

Immunoprecipitation: PBK Antibody [NBP3-12989]

Immunoprecipitation: PBK Antibody [NBP3-12989] - Immunoprecipitation of PBK protein from A431 whole cell extracts using 5 ug of PBK antibody (NBP3-12989). Western blot analysis was performed using PBK antibody (NBP3-12989) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
PBK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PBK Antibody [NBP3-12989] -

PBK antibody detects PBK protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PBK protein stained by PBK antibody (NBP3-12989) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for PBK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBK

PBK encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

Alternate Names

Cancer/testis antigen 84, CT84MAPKK-like protein kinase, EC 2.7.12.2, FLJ14385, Nori-3PDZ-binding kinase, PDZ binding kinase, serine/threonine protein kinase, Spermatogenesis-related protein kinase, SPKT-LAK cell-originated protein kinase, TOPKlymphokine-activated killer T-cell-originated protein kinase

Gene Symbol

PBK

Additional PBK Products

Product Documents for PBK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PBK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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