PCBP1 Antibody (1G2) - Azide and BSA Free

Novus Biologicals | Catalog # H00005093-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G2

Format

Azide and BSA Free
View all PCBP1 Primary Antibodies »

Product Specifications

Immunogen

PCBP1 (AAH39742, 181 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQT

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23285117).

Specificity

PCBP1 (1G2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PCBP1 Antibody (1G2) - Azide and BSA Free

Western Blot: PCBP1 Antibody (1G2) [H00005093-M01]

Western Blot: PCBP1 Antibody (1G2) [H00005093-M01]

Western Blot: PCBP1 Antibody (1G2) [H00005093-M01] - Analysis of PCBP1 expression in transfected 293T cell line by PCBP1 monoclonal antibody (M01), clone 1G2.Lane 1: PCBP1 transfected lysate(37.5 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PCBP1 Antibody (1G2) [H00005093-M01]

Immunocytochemistry/ Immunofluorescence: PCBP1 Antibody (1G2) [H00005093-M01]

Immunocytochemistry/Immunofluorescence: PCBP1 Antibody (1G2) [H00005093-M01] - Analysis of monoclonal antibody to PCBP1 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: PCBP1 Antibody (1G2) [H00005093-M01]

ELISA: PCBP1 Antibody (1G2) [H00005093-M01]

ELISA: PCBP1 Antibody (1G2) [H00005093-M01] - Detection limit for recombinant GST tagged PCBP1 is 0.03 ng/ml as a capture antibody.

Applications for PCBP1 Antibody (1G2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10ug/mL

Western Blot

1-5 ug/mL

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCBP1

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

Alternate Names

Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, hnRNP E1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, nucleic acid binding protein sub 2.3, Nucleic acid-binding protein SUB2.3, poly(rC) binding protein 1, poly(rC)-binding protein 1

Entrez Gene IDs

5093 (Human)

Gene Symbol

PCBP1

OMIM

601209 (Human)

UniProt

AAH39742 (Human)

Additional PCBP1 Products

Product Documents for PCBP1 Antibody (1G2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PCBP1 Antibody (1G2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCBP1 Antibody (1G2) - Azide and BSA Free

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Protocols

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FAQs

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