Phospho-Tyrosine Antibody (179003)

R&D Systems | Catalog # MAB1676

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 179003
View all Phospho-Tyrosine Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled phospho-tyrosine synthetic peptide

Specificity

Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence.No cross‑reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phospho-Tyrosine Antibody (179003)

Detection of Phospho-Tyrosine antibody by Western Blot.

Detection of Phospho-Tyrosine by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.
Detection of Human Phospho-Tyrosine antibody by Simple WesternTM.

Detection of Human Phospho-Tyrosine by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 15 minutes, loaded at 0.2 mg/mL. Tyrosine-phosphorylated proteins were detected (as indicated) using 50 µg/mL of Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Phospho-Tyrosine antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

Phospho-Tyrosine in A431 Human Cell Line.

Phospho-Tyrosine was detected in immersion fixed A431 human epithelial carcinoma cell line treated with 50 ng/mL Recombinant Human EGF (left panel; 236-EG) or untreated (right panel) using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Tyrosine in A431 Human Cell Line.

Tyrosine was detected in Immersion fixed A431 human epithelial carcinoma cell line treated with 100μM Pervanadate (PV) for 10 minutes (left panel) or untreated (right panel) using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676) at 10 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Phospho-Tyrosine Antibody (179003)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) or Pervanadate

Immunoprecipitation

5 µg/500 µg cell lysate
Sample: A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies

Simple Western

50 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)

Western Blot

1 µg/mL
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB1676 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Phospho-Tyrosine

Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal cell cycle progression and oncogenic transformation. The role  of tyrosine phosphorylation is a matter of active and ongoing research. Specific antibodies that  recognize phosphorylated tyrosine residues are valuable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they are involved.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (179003)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-Tyrosine Antibody (179003)

For research use only

Related Research Areas

GDNF Family

Citations for Phospho-Tyrosine Antibody (179003)

Customer Reviews for Phospho-Tyrosine Antibody (179003) (2)

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Customer Images

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  • Antibody works
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 12/10/2024
    WB_Phospho tyrosine - Liver tissue
    Liver tissue lysates were immunoblotted with the Phospho-Tyrosine Antibody at concentration of 1 ug/ml. Multiple bands of phosphorylated proteins were observed.
    Phospho-Tyrosine Antibody (179003) MAB1676
  • Phospho-Tyrosine Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/28/2017
    Immunoprecipitation with antibody for target protein and Western Blotting detection of phospholation.
    Phospho-Tyrosine Antibody (179003) MAB1676

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Protocols

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