Phosphoserine phosphatase Antibody (3G12) - BSA Free

Novus Biologicals | Catalog # NBP1-04297

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G12

Format

BSA Free
View all Phosphoserine phosphatase Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Phosphoserine phosphatase (1-225aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Phosphoserine phosphatase Antibody (3G12) - BSA Free

Western Blot: Phosphoserine phosphatase Antibody (3G12)BSA Free [NBP1-04297]

Western Blot: Phosphoserine phosphatase Antibody (3G12)BSA Free [NBP1-04297]

Western Blot: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04297 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: Jurkat cell lysate. Lane 2: K562 cell lysate. Lane 3: HepG2 cell lysate. Lane 4: A549 cell lysate. Lane 5: MCF7 cell lysate
Immunocytochemistry/ Immunofluorescence: Phosphoserine phosphatase Antibody (3G12) - BSA Free [NBP1-04297]

Immunocytochemistry/ Immunofluorescence: Phosphoserine phosphatase Antibody (3G12) - BSA Free [NBP1-04297]

Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - A431 cells stained with NBP1-04297 (1:100). Alexa Fluor 488 - conjugated secondary antibody was used (green). DAPI was stained the cell nucleus (blue).

Applications for Phosphoserine phosphatase Antibody (3G12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphoserine phosphatase

Human phosphoserine phosphatase (HPSP), specific for D- and L- phosphoserine, has been identified in all human tissues. HPSP is a Mg(2+)-dependent phosphoserine phosphatase. The three dimensional structure of HPSP reveals the structural and functional role of the divalent cation in the active site of phosphatases. In particular, the complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important for the substrate recognition and hydrolysis.

Alternate Names

L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, phosphoserine phosphatase, PSPase, PSPEC 3.1.3.3

Gene Symbol

PSPH

UniProt

NP_004568 (Human)

Additional Phosphoserine phosphatase Products

Product Documents for Phosphoserine phosphatase Antibody (3G12) - BSA Free

Certificate of Analysis

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Product Specific Notices for Phosphoserine phosphatase Antibody (3G12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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