Polyoma Virus, Large T Antigen Antibody (PyLT) [CoraFluor™ 1]

Novus Biologicals | Catalog # NB100-2755CL1

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG2B Clone # PyLT
View all Polyoma Virus, Large T Antigen Primary Antibodies »

Product Specifications

Immunogen

Polyoma virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25278611).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for Polyoma Virus, Large T Antigen Antibody (PyLT) [CoraFluor™ 1]

Polyoma Virus, Large T Antigen Antibody (PyLT) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Polyoma Virus, Large T Antigen Antibody (PyLT) [CoraFluor™ 1]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-2755. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Polyoma Virus, Large T Antigen

The mouse polyoma virus (Py) is an oncogenic virus, which encodes for three early proteins, large, middle and small T (tumour) antigen. PyLT allows isolation of viral T antigens.

Additional Polyoma Virus, Large T Antigen Products

Product Documents for Polyoma Virus, Large T Antigen Antibody (PyLT) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Polyoma Virus, Large T Antigen Antibody (PyLT) [CoraFluor™ 1]

Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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