Porcine IL-8/CXCL8 Antibody

Catalog # Availability Size / Price Qty
MAB535
MAB535-SP
Porcine IL-8/CXCL8 Antibody in Data
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Product Details
Citations (2)
FAQs
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Reviews (1)

Porcine IL-8/CXCL8 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL8 or rhCXCL1, 2, 3, 5, 6, 7, 9, 10, 11, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12, 13, recombinant rat CXCL1, 2 or 3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 105115
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant porcine IL-8/CXCL8
Ala26-Gln104
Accession # CAA43461
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN)
Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody. View Larger

Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine CXCL8/IL-8 Monoclonal Antibody (Catalog # MAB535). The ND50is typically 0.2-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

CXCL8, also called Interleukin 8 (IL-8), neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI), is a neutrophil chemotactic and activating factor in the CXC chemokine family. Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes, an autocrine growth factor for melanoma cells, and an angiogenic chemokine.

References
  1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Product Datasheets

Citations for Porcine IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant
    Authors: C Martin, SD Waghela, S Lokhandwal, A Ambrus, J Bray, C Vuong, V Vinodkumar, PJ Dominowski, S Rai, D Mwangi, DL Foss, W Mwangi
    PLoS ONE, 2017;12(1):e0170504.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells.
    Authors: Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP
    J. Gen. Virol., 2004;85(0):1029-37.
    Species: Porcine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Isotype Controls

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Secondary Antibodies

Reviews for Porcine IL-8/CXCL8 Antibody

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Porcine IL-8/CXCL8 Antibody
By Anonymous on 10/18/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Porcine

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC. Paired with goat PC IL-8 antibody (AF535) as a detection antibody. A standard curve with recombinant porcine IL-8 from RnD Cat# 535-IN/CF is shown 2-25,000 pg/ml.