Porcine IL-8/CXCL8 Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Porcine
  • Specificity
    Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, rhCXCL13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, rmCXCL13, recombinant rat (rr) CXCL1, rrCXCL3/CINC2 alpha, or rr3/CINC-2 beta is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 105105
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant porcine IL-8/CXCL8
    Ala26-Gln104
    Accession # CAA43461
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) under non-reducing conditions only
    • Porcine IL-8/CXCL8 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Porcine IL-8/CXCL8 Antibody (Catalog # MAB5351)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Porcine IL-8/CXCL8 Biotinylated Antibody (Catalog # BAF535)
  • ELISA Standard
     
    Recombinant Porcine IL-8/CXCL8 Protein (Catalog # 535-IN)
  • Neutralization
    Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by
IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody.
Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine
IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND50 is typically 1-6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8

Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro.

  • References:
    1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
  • Long Name:
    Interleukin 8
  • Entrez Gene IDs:
    3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
  • Alternate Names:
    3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Species
Applications
Sample Type
  1. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
    Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
    Intensive Care Med, 2007;33(6):1085-93.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  2. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
    Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
    Cytometry A, 2006;69(5):391-5.
    Species: Porcine
    Sample Type: Serum
    Application: Luminex Development
  3. Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
    Authors: Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E
    Anesthesiology, 2004;100(4):861-70.
    Species: Porcine
    Sample Type: Plasma
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG1 Isotype Control

Ctrl MAB002R 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Porcine IL-8/CXCL8 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Porcine IL-8/CXCL8 Antibody.

We have 1 review tested in 1 species: Porcine.
We have 1 review tested in 1 application: ELISA.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Porcine
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
ELISA Porcine IL-8/CXCL8 Antibody MAB5351
Enlarge
Excellent
 ELISA Porcine Anonymous 11/08/2017
Show
Image Details
ELISA Porcine IL-8/CXCL8 Antibody MAB5351
ELISA: Porcine IL-8/CXCL8 Antibody [MAB5351]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesPorcine

Other Experimental Details

Other Experimental DetailsThe antibody MAB5351 was used as the capture antibody in a sandwich ELISA for Porcine IL-8 along with the detection antibody, AF535. The calibrator for the assay was recombinant porcine IL-8, 535-IN. It was sensitive and specific.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information