Porcine IL-8/CXCL8 Antibody

Catalog # Availability Size / Price Qty
AF535
AF535-SP
Porcine IL-8/CXCL8 Antibody in Data
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Porcine IL-8/CXCL8 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human IL-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant porcine IL-8/CXCL8
Ala26-Gln104
Accession # CAA43461
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN)
Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL‑8 Antibody. View Larger

Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL‑8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND50is typically 1.5-7.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro-inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro.

References
  1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Product Datasheets

Citations for Porcine IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Matrine inhibits IL-1? secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome
    Authors: P Sun, N Sun, W Yin, Y Sun, K Fan, J Guo, A Khan, Y He, H Li
    Vet. Res., 2019;50(1):53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Exposure to hog barn dust alters airway epithelial ciliary beating.
    Authors: Wyatt TA, Sisson JH, Von Essen SG, Poole JA, Romberger DJ
    Eur. Respir. J., 2008;31(6):1249-55.
    Species: Porcine
    Sample Types: Complex Sample Type
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Porcine IL-8/CXCL8 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Porcine IL-8/CXCL8 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Porcine IL-8/CXCL8 Antibody
By Anonymous on 10/18/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Porcine

After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC). Paired with biotinylated IL-8 antibody (MAB535) as a capture reagent. A standard curve with recombinant porcine IL-8 from R&D (Cat# 535-IN/CF) is shown (2-25,000 pg/ml).


Porcine IL-8/CXCL8 Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Porcine Serum,Serum and Plasma Species: Porcine

The antibody MAB5351 was used as the capture antibody in a sandwich ELISA for Porcine IL-8 along with the detection antibody, AF535. The calibrator for the assay was recombinant porcine IL-8, 535-IN. It was sensitive and specific.