PRA1 Antibody (2A4) - Azide and BSA Free

Novus Biologicals | Catalog # H00010567-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2A4

Format

Azide and BSA Free
View all PRA1 Primary Antibodies »

Product Specifications

Immunogen

RABAC1 (NP_006414.1, 1 a.a. ~ 77 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAQKDQQKDAEAEGLSGTTLLPKLIPSGAGREWLERRRATIRPWSTFVDQQRFSRPRNLGELCQRLVRNVEYYQSN

Specificity

RABAC1 - Rab acceptor 1 (prenylated) (2A4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PRA1 Antibody (2A4) - Azide and BSA Free

Sandwich ELISA: PRA1 Antibody (2A4) [H00010567-M01] - Detection limit for recombinant GST tagged RABAC1 is approximately 0.3ng/ml as a capture antibody.

Applications for PRA1 Antibody (2A4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23813961)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRA1

PRA1 (prenylated Rab acceptor) is a general regulator of Rab proteins. It has been shown that PRA1 interacts with Rab proteins and with VAMP2. Therefore PRA1 is probably an important factor for membrane traffic, linking together the function of Rab proteins and SNAREs (1). Human cells contain more than 60 small G proteins of the Rab family, which are localized to the surfaces of distinct membrane compartments and regulate transport vesicle formation, motility, docking and fusion. Prenylated Rabs also occur in the cytosol bound to GDI (guanine nucleotide dissociation inhibitor), which binds to Rabs in their inactive state (2).

Alternate Names

PRA1 family protein 1, PRA1PRA1 domain family 1, PRAF1prenylated Rab acceptor 1, prenylated Rab acceptor protein 1, Rab acceptor 1 (prenylated), YIP3

Entrez Gene IDs

10567 (Human)

Gene Symbol

RABAC1

UniProt

NP_006414 (Human)

Additional PRA1 Products

Product Documents for PRA1 Antibody (2A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PRA1 Antibody (2A4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PRA1 Antibody (2A4) - Azide and BSA Free

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Protocols

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FAQs

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