Rabbit TNF-alpha Antibody (610331)

Name: Rabbit TNF-alpha Antibody (610331)
Brand: R&D Systems
SKU: MAB5670
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # MAB5670

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Product Specifications
Scientific Data
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Key Product Details

Species Reactivity

Rabbit

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 610331

View all TNF-alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rabbit TNF-alpha
Leu79-Leu234
Accession # NP_001075732

Specificity

Detects rabbit TNF-alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant TNF-alpha from cotton rat, cat, cow, dog, guinea pig, horse, human, mouse, pig, rat, or rhesus is observed. No cross-reactivity with recombinant human (rh) 4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1 beta 2, rhLymphotoxin alpha 2 beta 1, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, rhVEGI, recombinant mouse (rm) 4-1BB Ligand, rmBAFF, rmFas Ligand, rmGITR Ligand, rmLIGHT, rmOX40 Ligand, rmTRAIL, rmTRAIL-2L, rmTRANCE, or rmTWEAK is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rabbit TNF-alpha Antibody (610331)

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Rabbit TNF‑ alpha Antibody.

Recombinant Rabbit TNF-a (Catalog # 5670-TG) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rabbit TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit TNF-a Monoclonal Antibody (Catalog # MAB5670). The ND₅₀ is typically 0.4-2.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Applications for Rabbit TNF-alpha Antibody (610331)

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.4-2.4 µg/mL in the presence of 0.25 ng/mL Recombinant Rabbit TNF‑ alpha and the metabolic inhibitor actinomycin D (1 µg/mL).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF-alpha

Tumor necrosis factor (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily and family. The 26 kDa type II transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Rabbit TNF-alpha is 235 amino acids (aa) in length and contains a 35 aa cytoplasmic domain, a 21 aa transmembrane region, and a 179 extracellular domain (ECD). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. Rabbit TNF-alpha is 79% and 78% aa identical to human and mouse TNF-alpha, respectively. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)