Raf-1 Antibody (1H4) - Azide and BSA Free

Name: Raf-1 Antibody (1H4) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005894-M03
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # H00005894-M03

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1H4

Format

Azide and BSA Free

View all Raf-1 Primary Antibodies »

Product Specifications

Immunogen

RAF1 (AAH18119, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDF

Specificity

RAF1 - v-raf-1 murine leukemia viral oncogene homolog 1 (1H4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Raf-1 Antibody (1H4) - Azide and BSA Free

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03]

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody (M03), clone 1H4. Lane 1: RAF1 transfected lysatE (73.1 KDa). Lane 2: Non-transfected lysate.

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03]

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi or non-transfected control. Blot probed with H00005894-M03. GAPDH (36.1 kDa) used as loading control.

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03]

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in HeLa.

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03]

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in rat brain.

Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03]

Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between BAD and RAF1. HeLa cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-RAF1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction compl

Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03]

Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-RAF1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction

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Applications for Raf-1 Antibody (1H4) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500

Application Notes

Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Raf-1

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Entrez Gene IDs

5894 (Human)

Gene Symbol

RAF1

UniProt

AAH18119 (Human)

Additional Raf-1 Products

Product Documents for Raf-1 Antibody (1H4) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for Raf-1 Antibody (1H4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.