Rat ALK-7 Antibody (118311)

R&D Systems | Catalog # MAB5771

R&D Systems
Discontinued Product
MAB5771 has been discontinued. View all ALK-7 products.

Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 118311
View all ALK-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat ALK‑7
Leu26-Arg108
Accession # NP_620790

Specificity

Detects rat ALK-7 in Western blots. In Western blots, no cross-reactivity with recombinant human Activin R1B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Rat ALK-7 Antibody (118311)

Detection of ALK‑7 in PC-12 cells by Flow Cytometry

PC-12 cells were stained with Mouse Anti-Rat ALK‑7 Monoclonal Antibody (Catalog # MAB5771, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat ALK-7 Antibody (118311)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: PC-12 rat adrenal pheochromocytoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Rat ALK‑7 Fc Chimera (Catalog # 577-A7)

Reviewed Applications

Read 1 review rated 4 using MAB5771 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ALK-7

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. ALK-7 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. Rat ALK-7 expression is restricted to the adult central nervous system, suggesting a possible role of ALK-7 during postnatal maturation and maintenance of several neuronal subpopulations in the cerebellum.

References

  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Ryden, M. et al. (1996) J. Biol. Chem. 271:30603.

Long Name

Activin Receptor-like Kinase 7

Alternate Names

Activin RIC, ACVR1C, ACVRLK7, ALK7

Entrez Gene IDs

130399 (Human); 245921 (Rat)

Gene Symbol

ACVR1C

UniProt

NP_620790

Additional ALK-7 Products

Product Documents for Rat ALK-7 Antibody (118311)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat ALK-7 Antibody (118311)

For research use only

Customer Reviews for Rat ALK-7 Antibody (118311) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23830891
    Species: Rat
    Verified Customer | Posted 02/25/2015

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