Rat beta 2-Microglobulin Alexa Fluor® 700-conjugated Antibody

Catalog #: AF3864N Datasheet / COA / SDS

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AF3864N-100UG

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Rat beta 2-Microglobulin Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat beta ₂-Microglobulin ( beta ₂M) in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Î²₂-Microglobulin
Ile21-Met119
Accession # P07151

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: beta 2-Microglobulin

beta ₂-Microglobulin ( beta ₂M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta ₂M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta ₂M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat beta ₂M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.

Entrez Gene IDs

567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)

Alternate Names

B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin