RhoGDI Antibody (OTI1F2)

Novus Biologicals | Catalog # NBP2-02467

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F2
View all RhoGDI Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoGDI Antibody (OTI1F2)

Western Blot: RhoGDI Antibody (1F2) [NBP2-02467] Analysis of extracts (35ug) from 9 different cell lines by using anti-RhoGDI monoclonal antibody.
Flow Cytometry: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Flow Cytometry: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Flow Cytometry: RhoGDI Antibody (1F2) [NBP2-02467] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RhoGDI antibody, and then analyzed by flow cytometry.
Western Blot: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Western Blot: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Western Blot: RhoGDI Antibody (1F2) [NBP2-02467] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoGDI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoGDI.
Flow Cytometry: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Flow Cytometry: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Flow Cytometry: RhoGDI Antibody (1F2) [NBP2-02467] - Analysis of Hela cells, using anti-RhoGDI antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Flow Cytometry: RhoGDI Antibody (OTI1F2) [NBP2-02467]

Flow Cytometry: RhoGDI Antibody (1F2) [NBP2-02467] - Analysis of Jurkat cells, using anti-RhoGDI antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for RhoGDI Antibody (OTI1F2)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:200-1000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoGDI

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Alternate Names

GDIA1, MGC117248, Rho GDI 1, Rho GDP dissociation inhibitor (GDI) alpha, rho GDP-dissociation inhibitor 1, RHOGDI, Rho-GDI alpha, RHOGDI-1

Entrez Gene IDs

396 (Human)

Gene Symbol

ARHGDIA

Additional RhoGDI Products

Product Documents for RhoGDI Antibody (OTI1F2)

Certificate of Analysis

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Product Specific Notices for RhoGDI Antibody (OTI1F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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