Key Product Details

Species Reactivity

Human, Mouse, Chinese Hamster

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all RNF2 Primary Antibodies »

Product Specifications

Immunogen

RNF2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RNF2 protein. (Uniprot: Q99496)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RNF2 from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RNF2 homologues from other sources has not been determined.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Affinity purified Anti-RNF2 antibody is directed against human RNF2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for RNF2 Antibody - BSA Free

Western Blot: RNF2 Antibody [NBP1-77826]

Western Blot: RNF2 Antibody [NBP1-77826]

Western Blot: RNF2 Antibody [NBP1-77826] - Detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.
Immunocytochemistry/ Immunofluorescence: RNF2 Antibody [NBP1-77826]

Immunocytochemistry/ Immunofluorescence: RNF2 Antibody [NBP1-77826]

Immunocytochemistry/Immunofluorescence: RNF2 Antibody [NBP1-77826] - Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25C. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25C. Secondary antibody: Alexa Fluor488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
RNF2 Antibody

RNF2 Antibody

Western blot using Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B. Lane 1: 3T3 whole cell lysates

Applications for RNF2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:25000

Immunocytochemistry/ Immunofluorescence

1:300

Western Blot

1:500-1:2000
Application Notes
This product has been tested for use in ELISA, immunofluorescence, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to RING1B by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: RNF2

RING1B (also known as BAP1, DING, Polycomb-M33 interacting protein Ring1B, Ring finger protein 1b, Ring finger protein 2 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity.

Long Name

Ring Finger Protein 2

Alternate Names

DING, HIPI3, RING1B, RING2

Gene Symbol

RNF2

UniProt

NP_009143 (Human), Q99496 (Human)

Additional RNF2 Products

Product Documents for RNF2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RNF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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