ROCK2 Antibody - BSA Free

Name: ROCK2 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB100-593
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-593

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1320 and the C-terminus (residues 1388) of human ROCK2 using the number given in entry NP_004841.1 (GeneID 9475)

Specificity

NB100-593 is specific for human Rho Kinase alpha protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK2 Antibody - BSA Free

Western Blot: ROCK2 Antibody [NB100-593]

Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: ROCK2 Antibody [NB100-593]

Western Blot: ROCK2 Antibody [NB100-593] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-593. NB100-593 and NB100-592 were used at 5 ug/mg lysate to immunoprecipitate Rho Kinase Alpha.

Applications for ROCK2 Antibody - BSA Free

Application

Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:10000-1:25000

Application Notes

Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000 Immunoprecipitation: 2 to 5 ug/mg of lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ROCK2

The small GTPase Rho is implicated in cytoskeletal rearrangements including stress fiber and focal adhesion formation and in the transcriptional activation of c-fos serum response element. In vitro, Rho-kinase, which is activated by Rho, phosphorylates not only myosin light chain (MLC) (thereby activating myosin ATPase) but also myosin phosphatase, thus inactivating myosin phosphatase. Rho-kinase is involved in the formation of stress fibers and focal adhesions in fibroblasts.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Entrez Gene IDs

9475 (Human)

Gene Symbol

ROCK2

UniProt

O75116

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Product Specific Notices for ROCK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.