SA1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-299

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all SA1 Primary Antibodies »

Product Specifications

Immunogen

A 35 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 1 encoded in part by exons 35 and 36 (LocusLink ID 10274).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SA1 Antibody - BSA Free

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody NB100-299 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-299]

Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-299]

Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-299] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.
Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.
Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: SA1 Antibody [NB100-299]

Immunoprecipitation: SA1 Antibody [NB100-299]

Immunoprecipitation: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody NB100-299 (lot NB100-299-3) used for IP at 6 ug per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-299-2). For blotting immunoprecipitated SA1, NB100-299 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Western Blot

1:1000-1:10000
Application Notes
NB 100-299 may be used for Western Blot where a band at ~145 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SA1

SA1 is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Alternate Names

cohesin subunit SA-1, nuclear protein stromal antigen 1, SA1, stromal antigen 1

Gene Symbol

STAG1

Additional SA1 Products

Product Documents for SA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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