Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 758726
View all Semaphorin 4D/CD100 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Semaphorin 4D/CD100
Met22-Arg734
Accession # Q92854

Specificity

Detects human Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 4B, 4C, 4G, or recombinant mouse Semaphorin 4D is observed. In Western blots, no cross-reactivity with rhSemaphorin 4A, 6A, or 6C is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Semaphorin 4D/CD100 Antibody (758726) [DyLight 488]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Semaphorin 4D/CD100

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Gene Symbol

SEMA4D

Additional Semaphorin 4D/CD100 Products

Product Documents for Semaphorin 4D/CD100 Antibody (758726) [DyLight 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Semaphorin 4D/CD100 Antibody (758726) [DyLight 488]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans

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