Skp1 Antibody (1H8) - Azide and BSA Free

Novus Biologicals | Catalog # H00006500-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Bovine

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H8

Format

Azide and BSA Free
View all SKP1 Primary Antibodies »

Product Specifications

Immunogen

SKP1A (NP_008861, 53 a.a. ~ 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVGSTQFCL

Specificity

SKP1A - S-phase kinase-associated protein 1A (p19A)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Skp1 Antibody (1H8) - Azide and BSA Free

Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01] - SKP1A monoclonal antibody (M01), clone 1H8 Analysis of SKP1A expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (1H8) [H00006500-M01]

Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (1H8) [H00006500-M01]

Immunocytochemistry/Immunofluorescence: Skp1 Antibody (1H8) [H00006500-M01] - Analysis of monoclonal antibody to SKP1A on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01] - Analysis of SKP1 expression in transfected 293T cell line by SKP1A monoclonal antibody (M01), clone 1H8.Lane 1: SKP1 transfected lysate(18.1 KDa).Lane 2: Non-transfected lysate.

Applications for Skp1 Antibody (1H8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SKP1

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Long Name

S-phase Kinase Associated Protein 1

Alternate Names

OCP2, p19a, p19Skp1, SKP1A

Entrez Gene IDs

6500 (Human)

Gene Symbol

SKP1

OMIM

601434 (Human)

UniProt

NP_008861 (Human)

Additional SKP1 Products

Product Documents for Skp1 Antibody (1H8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Skp1 Antibody (1H8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Skp1 Antibody (1H8) - Azide and BSA Free

Customer Reviews for Skp1 Antibody (1H8) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Skp1 Antibody (1H8) - Azide and BSA Free and earn rewards!

Have you used Skp1 Antibody (1H8) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies