STAT1 [p Tyr701] Antibody - BSA Free

Name: STAT1 [p Tyr701] Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-77825
Price: 619 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-77825

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This Protein A purified STAT1 [p Tyr701] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein. (Uniprot: Q99K94)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with STAT1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against STAT1 from human sources as 90% homology is noted. Cross-reactivity with STAT1 from other sources has not been determined.

Modification

p Tyr701

Specificity

This antibody is specific for mouse STAT1 protein phosphorylated at Y701. A BLAST analysis was used to suggest cross-reactivity with STAT1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against STAT1 from human sources as 90% homology is noted. Cross-reactivity with STAT1 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was Protein A purified from monospecific antiserum

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for STAT1 [p Tyr701] Antibody - BSA Free

Western Blot: STAT1 [p Tyr701] Antibody [NBP1-77825]

Western Blot: STAT1 [p Tyr701] Antibody [NBP1-77825] - Shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-a (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.

Applications for STAT1 [p Tyr701] Antibody - BSA Free

Application

Recommended Usage

ELISA

1:25000

Western Blot

1:500-1:2000

Application Notes

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: STAT1

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Gene Symbol

STAT1

UniProt

NP_033309 (Mouse), Q99K94 (Mouse)

Additional STAT1 Products

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Product Specific Notices for STAT1 [p Tyr701] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.