SV2A Antibody

Novus Biologicals | Catalog # NB100-41402

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SV2A Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TVELYPSDKRTTA corresponding to internal region according to NP_055664.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SV2A Antibody

Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NB100-41402]

Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NB100-41402]

Immunocytochemistry/Immunofluorescence: SV2A Antibody [NB100-41402] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NB100-41402]

Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NB100-41402]

Immunocytochemistry/Immunofluorescence: SV2A Antibody [NB100-41402] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for SV2A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:1000

Reviewed Applications

Read 1 review rated 4 using NB100-41402 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SV2A

SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.

Long Name

Synaptic Vesicle Glycoprotein 2A

Alternate Names

KIAA0736, SV2

Entrez Gene IDs

9900 (Human); 64051 (Mouse); 117559 (Rat)

Gene Symbol

SV2A

UniProt

NP_055664.2 (Human)

Additional SV2A Products

Product Documents for SV2A Antibody

Certificate of Analysis

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Product Specific Notices for SV2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SV2A Antibody (1)

4 out of 5
1 Customer Rating
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  • Name: Nadir Mahmood
    Application: Western Blot
    Sample Tested: Neuro 2A cells
    Species: Other
    Verified Customer | Posted 05/17/2010

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Protocols

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